FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154261
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT50470.43724946091048733No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA24240.21000449638340027No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT22780.19735571071014266No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC21600.1871327195495646No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG21150.18323412122561536No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19890.1723180459185574No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT19310.16729318585657837No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA18870.1634812230509391No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18120.15698355917769033No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT17590.15239187670726118No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT17500.15161215704247133No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA16700.14468131557767266No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT16630.14407486694950275No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG16090.13939654896076364No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA15240.13203252990441502No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA14520.12579477258609623No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14400.1247551463663764No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA14400.1247551463663764No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14300.12388879118327657No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT14230.12328234255510669No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT14200.12302243600017673No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13520.11713122075509784No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG13290.11513860383396822No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG12900.11175981861987887No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT12880.1115865475832589No Hit
GTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCTGTATCAGTCT12710.11011374377198918No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG12680.10985383721705923No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG11880.10292299575226055No Hit
GACACATACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGC11560.10015065916634107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA14550.0124.17954
GAAACAC4950.0116.8039864
AAACACG5150.0113.666325
TAGAAAC5500.0109.0893862
AACACGT2650.089.995776
TAGTAAC20250.088.887652
CACGCAC1700.080.782123
TAACAAG23250.078.330625
GCACGCA1850.078.149352
ACACGTG3200.076.776177
AGCACGC1950.074.1545641
ACAAGAG24400.073.148377
AGTAACA24850.073.005893
CGCACAA300.001934589772.51158145
CTCGCTG1000.072.30071
CGCACTT1900.072.2693255
ACGCACT2100.065.389374
AACACGA5350.063.488946
ACACGAG5700.059.582757
GAGCATC1850.058.5967566
Produced by FastQC (version 0.11.5)