FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039272
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT28250.2718248928095821No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21160.20360406130445155No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA17800.17127373777028534No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC15300.14721843752164976No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14280.13740387502020646No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13300.1279741973227413No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC12690.12210470406207423No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG12290.11825585602229254No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC12150.11690875920836895No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA11980.11527299879146172No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA11640.11200147795764727No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA10820.10411133947609481No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10640.10237935785819305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA10250.0114.918094
AAACACG4050.0112.411365
GAAACAC4200.0108.396674
CACGCAC1200.0102.38453
TAGAAAC4950.097.8360442
GCACGCA1350.096.37582
AGCACGC1450.089.733521
CGCACTT1450.089.707595
TAACAAG14750.080.348275
ACACGTG1750.078.4547657
ACGTGCA2350.076.8955842
AACACGT1800.076.279146
AACACGA4200.075.7056056
ACACGAG4650.068.375977
TAGTAAC17950.067.650982
CACGTGC3350.066.890911
ACGCACT1850.066.405174
CATAAGG651.1130525E-555.6041031
GGATCGT400.006122348454.2141
AGTAACA22950.053.219473
Produced by FastQC (version 0.11.5)