FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259111
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45220.35914228372240414No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT34540.27432053250269434No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA23260.18473351436052898No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT22400.17790329843834263No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT19920.1582068618255261No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA19770.1570155450949122No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT16780.13326863159800845No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC16490.1309654192521549No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15990.1269943634834419No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC15780.12532652006058242No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG15630.12413520332996852No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC14330.11381045833131472No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT14200.11277798383144934No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT13890.11031592925484726No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13560.10769503244749667No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC13400.10642429460150853No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA13210.10491529340939758No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA12960.10292976552504107No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT12960.10292976552504107No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT12690.10078539540993607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA100.007150578144.595291
TATACGT100.0071539795144.57232
CACGCAC6150.0122.230283
GCACGCA6150.0121.064612
AGCACGC6200.0120.107381
GAAACAC4150.0104.489324
CGCACTT7350.0103.237195
ACGCACT7600.098.8982244
AAACACG4750.091.283425
TAGTAAC17350.082.910342
GTAGTAA18600.079.682891
TAGAAAC5750.077.943332
ACGTGCA1550.074.617962
GAAGCGG1700.072.297651
GTAACAA20800.069.8395544
CAACGCA2000.068.659
CGGTGCC955.456968E-1268.481612
ACAACGC2050.066.975618
AGTAACA21600.066.92633
AAGCGGT651.4272337E-766.725682
Produced by FastQC (version 0.11.5)