FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083140
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30300.27974223092121053No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT24120.2226858947135181No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA24030.22185497719593036No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17680.16322913012168327No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA15600.14402570304854403No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15060.13904019794301753No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14570.1345163136805953No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT14380.13276215447679893No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14040.12962313274368964No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA13760.12703805602230553No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT13220.12205255091677901No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT13160.12149860590505382No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG12840.11854423250918626No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12320.11374337574090146No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG12110.11180456819986336No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12050.11125062318813818No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT11760.10857322229813321No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT11720.10820392562364976No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11240.1037723655298484No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT11170.10312609634950237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC1600.0122.003783
GTAACAA11450.0115.540694
AAACACG5500.0107.775595
GAAACAC5550.0106.809584
TAGAAAC6600.094.211532
GCACGCA2100.092.959552
CGCACTT2250.089.959035
ACGCACT2300.088.007474
AGCACGC2350.086.162861
TAGTAAC16000.081.79152
ACGTGCA3650.079.234942
CATAAGG1500.077.136271
TAACAAG17900.074.7115865
ACGTGTC300.00195834972.288515
GAGCATC2950.071.056716
CTCGCTG1350.069.636911
CACGTGC5200.069.53391
AACACGA6550.068.419436
AACACGT2300.065.996456
AGCCACG451.1920124E-464.46506145
Produced by FastQC (version 0.11.5)