FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066084
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT44780.42004194791404803No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT24370.22859361926452323No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA21800.20448670086034496No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19960.18722727289782043No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA16820.1577736838748166No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT15000.14070185839014562No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT14530.1362932001605877No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14400.1350737840545398No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG13120.12306722547191402No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT12850.12053459202089141No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA12830.1203469895430379No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT12770.11978418210947729No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC12710.11922137467591672No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG11860.1112482693671418No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC11260.10562019503153597No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC10980.1029937603415866No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT10910.10233715166909924No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA10730.1006487293684175No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG10700.1003673256516372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA100.007151851144.585662
GCACGCA7300.0124.7793962
CACGCAC7350.0123.918923
AGCACGC7700.0118.2973561
CGCACTT9300.0102.58995
ACGCACT9200.0100.5718844
GAAACAC4300.099.183174
AAACACG4350.098.033925
GTAACAA16700.084.83874
ACGTGCA2600.080.634312
TAGTAAC17950.079.340872
CGGTGCC950.076.097722
TAGAAAC6250.074.027862
GTAGTAA20050.073.194241
TAACAAG19500.072.64995
AACACGT1800.072.279256
CAACGCA2300.072.275859
CACGTGC5100.070.875321
GAAGCGG1450.069.7999651
ACAACGC2400.069.270878
Produced by FastQC (version 0.11.5)