FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080553
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT32550.3012346455935063No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27770.25699803711618036No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA21480.1987871025299083No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC17210.15927029955957736No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC15900.14714687757102152No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA14770.13668926929081684No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14700.13604145284868027No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA14670.13576381723062173No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT13720.12697202265876825No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13560.1254912993624561No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13290.1229925787999293No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12890.11929077055914888No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA12740.11790259246885623No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG12650.11706968561468063No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12000.11105424722341246No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG11900.11012879516321736No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT11470.1061493513043784No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG11340.10494626362612477No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT11050.10226245265155898No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA10990.10170718141544191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA13000.0117.303594
GAAACAC5000.0109.853984
AAACACG5150.0105.241255
CACGCAC1300.0100.083323
AGCACGC1250.098.322291
GCACGCA1350.091.0307162
TAGAAAC6250.090.21682
TAGTAAC18050.086.1061252
TAACAAG19250.080.336885
AACACGT2000.075.8789446
AGTAACA22350.070.180233
AACACGA5650.069.068066
GAGCATC2950.066.141456
CGCACTT1750.066.071465
ACACGAG6100.063.9699137
ACGTGCA2750.063.088672
ACACGTG2300.062.8367927
ACAACAG3150.061.967831
ACAAGAG25950.059.592047
ACGCACT2150.057.145584
Produced by FastQC (version 0.11.5)