FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143342
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC40810.35693606987235665No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT39540.3458282823512125No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA32130.28101827799556034No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24880.2176076799417847No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT23310.20387600560462224No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21690.1897070167981234No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT20230.17693743429350098No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA19230.1681911449067733No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT18300.16005709577711655No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG17160.150086325876247No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA16730.1463254214399541No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16310.14265197989752848No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG15940.13941585282443925No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC15320.13399315340466808No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA14840.12979493449903878No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14670.12830806530329508No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC14420.12612149295661315No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC14350.12550925269954222No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG14010.1225355143080548No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG13860.12122357090004565No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT13650.11938685012883284No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG13490.11798744382695642No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC13170.11518863122320355No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG12780.11177757836237977No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT12580.11002832048503423No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT12540.10967846890956512No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC11960.10460562106526305No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT11720.10250651161244843No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT11600.10145695688604109No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG11500.10058232794736832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC7750.0119.430191
CACGCAC7800.0118.617853
GCACGCA7900.0117.142012
CGCACTT9500.0101.186995
ACGCACT9700.098.355544
GTAACAA18550.091.1734854
GAAACAC3000.089.1409154
AAACACG3050.087.679595
TAACAAG20000.084.202025
TAGTAAC21000.081.249832
ACAACGC2000.075.880278
AGTAACA22800.075.136013
CGGAGCG406.756066E-572.2985842
ACGTGCA2350.067.683782
GTAGTAA25750.067.39691
CAACGCA2300.062.8408059
GAAGCGG1750.061.981071
AACACGT1650.061.3200766
AACACGA2950.061.280721
TTAGTAC2400.060.2356223
Produced by FastQC (version 0.11.5)