FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248857
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT33840.2709677729315686No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT30800.24662551437034025No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT27670.22156259683854918No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT19460.15582248407944224No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC18110.145012599521002No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC17970.14389157445568226No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC17890.143250988704071No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT17730.14196981720084845No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT17650.14132923144923717No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG17640.14124915823028578No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC16660.13340198277304766No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC16240.1300389075770885No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA15470.12387326971782998No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC15290.12243195177670463No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT14330.11474492275736935No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA14250.11410433700575807No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC14180.11354382447309819No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT13880.11114162790455592No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG13600.10889957777391647No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT13580.10873943133601364No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA13560.10857928489811085No Hit
AACCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATG13210.10577672223481152No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC12950.10369481854207487No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA12930.10353467210417205No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG12830.10273393991465797No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA12600.10089225587877554No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACA100.0070797685145.07736145
CACGCAC5150.0120.719733
GCACGCA5500.0113.055692
TTATCGG203.8580853E-4108.80803145
CGCACTT5950.0106.8927465
AGCACGC5900.0106.6291961
ACGCACT6000.0103.601144
AGCCACG259.367416E-487.046425145
GAAACAC3300.081.041064
GTAGTAA11900.075.350071
AAACACG3550.075.32795
ACAACGC801.0186341E-1072.268288
TAGAAAC3900.070.4491352
AACACGT1650.070.081146
GCACTTT10100.069.408956
TAGTAAC12950.068.673942
ATATAGG1900.068.486653
ACGTGCC2200.065.730052
ACACGTG1850.062.50487
ATCACCG350.003559177562.176014145
Produced by FastQC (version 0.11.5)