FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085462
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT32200.29664787896766537No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG20130.1854509876900343No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT19170.17660682732329644No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG18820.1733823938562566No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA16830.15504918642937293No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG16460.1516404996213594No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA15590.1436254792890032No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT14880.13708448568443668No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14530.13386005221739683No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14100.12989860538646217No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA13650.1257529052145538No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC13160.12123869836069803No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA13090.12059381166729007No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT12920.11902765826901356No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT12880.11865915158706616No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC12870.1185670249165793No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA12840.11829064490511873No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT12730.11727725152976337No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA12590.11598747814294742No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA12150.1119339046415259No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT11840.10907797785643349No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA11820.10889372451545977No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11770.1084330911630255No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT11700.10778820446961754No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG11670.10751182445815699No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11620.10705119110572271No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG11570.10659055775328846No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT11540.10631417774182791No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC11310.1041952643206303No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA11300.10410313765014344No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11220.10336612428624861No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA10910.1005101975011562No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT10880.10023381748969562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA11750.0126.718754
CACGCAC1550.0102.608343
GAAACAC4350.099.695034
GCACGCA1650.096.402992
TAGTAAC15700.095.7889562
AAACACG4950.087.60275
AGCACGC1850.085.996911
TAGAAAC5450.082.25212
TAACAAG18350.081.921655
CGCACTT2100.079.1553045
CATAAGG755.638867E-1177.136631
AGTAACA19850.075.752073
ACGTGCA3000.074.712322
AACACGT2050.074.028146
GCCGACC406.6468245E-572.53652145
ACAAGAG21450.069.061817
GAGCGTT851.7462298E-1068.02724
TTTCCCG554.0497016E-665.94229145
ACACGTG2200.065.692947
ACGCACT2600.063.9390224
Produced by FastQC (version 0.11.5)