FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216317
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT37630.3093765852158607No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26870.22091280480335307No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT26810.22041951234752127No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25840.2124446176449067No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA20330.16714392711768394No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT19550.16073112519187022No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC18240.14996090657287534No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA18180.1494676141170435No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA16090.13228459357223488No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC15390.12652951492086356No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT15160.12463856050684155No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG14910.12258317527420895No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC14880.12233652904629302No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC14770.12143215954393469No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA14690.12077443626949225No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT14350.11797911235311188No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC14290.11748581989728006No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT14170.11649923498561641No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG13870.11403277270645729No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC13830.11370391106923607No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT13500.11099080256216103No Hit
GTATAGCACCGGTCTTATTCTCCAATAAAATAGCCAGACTGGGAAAAGGA13480.1108263717435504No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC13470.1107441563342451No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC13450.1105797255156345No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC13380.11000421765049737No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA13240.1088532019202231No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA12960.10655117045967459No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT12770.1049890776828738No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC12710.10449578522704196No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA12460.10244039999440936No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC12370.10170046131066163No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT12290.10104273803621917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC4450.0121.841881
CACGCAC4400.0121.568413
GCACGCA4650.0116.596562
ACGCACT4900.0109.154484
CGCACTT5150.0108.066095
AAACACG2950.093.103825
GAAACAC3200.085.8300864
AACACGT1500.081.911666
GTAGTAA13550.074.693721
GCACTTT7650.073.6921546
ACGTGCA1200.072.289872
GAAGCGG1550.069.960821
TAGTAAC14100.069.72642
TAGAAAC4150.067.935062
ACACGTG1900.064.66717
TTTCCCG808.059942E-963.46528145
AAGCGGT851.3358658E-859.5328332
GTACTAT2650.054.5471656
AGCCCGC400.00612008154.2196351
CGGTGCC805.993661E-754.2174032
Produced by FastQC (version 0.11.5)