FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328242
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT35280.26561424800601097No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT27080.20387851009078164No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG23030.17338707855947935No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC22300.16789109213531872No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA22060.16608419248901932No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT22000.16563246757744446No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG19380.14590714643867608No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT19310.14538013404183878No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA18900.1422933471460773No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT18440.13883012282400345No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA18240.13732437311875398No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17860.13446344867877993No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA17410.13107551184196856No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT17280.13009677453355636No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT17010.12806401243146956No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA16960.12768757500515718No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA16920.1273864250641073No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT16490.12414906319782087No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC16470.12399848822729594No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT16200.12196572612520912No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC16040.12076112636100951No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG15790.11887893922944764No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG15590.11737318952419816No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG15560.11714732706841072No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT14930.11240421549687481No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA14740.1109737532768878No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG14020.1055530543379896No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT14000.10540247936746466No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA13830.1041225921180026No Hit
GTAAGATGTTTGCCTTTTCCCCCGGTTTCAGCTTTTCAAGTTCTTCAATA13450.10126166767802855No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC100.00714958144.602341
CGCTATC100.00716168144.520584
GTAACAA11650.0115.9886254
AAACACG4650.0105.6635
CACGCAC2150.0104.212823
GAAACAC4800.0102.368754
AGCACGC2250.099.614951
GCACGCA2350.095.3616262
TAGTAAC14600.093.08612
CGCACTT2550.090.6727455
CCGCACT259.5117453E-486.712344
CGGGACT259.5145946E-486.705815
TAGAAAC6050.084.83652
ACGCACT2950.078.384054
ACAAGAG17650.076.5475167
GAGCATC2650.073.615376
AACACGA4900.072.252126
ACGTGCA2450.070.814952
AACACGT2050.066.965386
ACACGAG5300.066.7966167
Produced by FastQC (version 0.11.5)