FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931104
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT22250.23896363886311303No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT15410.16550245729800322No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT14110.15154053682510224No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12590.1352158298106334No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC12050.12941626284496682No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA11920.12802007079767672No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11800.1267312781386397No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC11510.12361669587930028No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA11180.12007251606694848No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA10710.1150247448190535No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC10600.11384335154826959No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT10540.11319895521875106No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA10440.11212496133622023No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT10270.11029917173591779No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG10150.10901037907688077No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG10150.10901037907688077No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT10110.10858078152386844No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT10080.10825858335910918No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC10000.10739938825308451No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT9980.10718458947657834No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC9970.10707719008832527No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG9930.10664759253531293No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG9850.10578839742928824No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT9820.10546619926452899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT1700.0123.2844245
CACGCAC1750.0119.768453
GTAACAA8450.0117.171774
ACGCACT1850.0113.288394
GCACGCA1950.0107.5191962
AGCACGC2100.099.844631
AAACACG3300.096.3602455
CGCCGTT259.37638E-487.02369145
ACGTGCA1800.084.3469542
GAAACAC3900.083.3886644
TAGAAAC4100.079.350792
AACACGT1400.077.428176
TAACAAG13100.075.580265
TAGTAAC13500.075.510612
TGGCGTG300.001933607272.51975145
AACACGA3150.066.5308766
ACACGTG1650.065.696637
CACGTGC3150.061.9725271
ACACGAG3400.061.6388977
AGTAACA17550.058.066343
Produced by FastQC (version 0.11.5)