FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054219
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT86120.8169080617974065No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA34640.32858447817768416No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG29330.2782154372099156No Hit
AGCACGCACTTTCTGTCTTCTAAAGGGCCGCCACATGCCAGGAGCTCAGG20250.19208532572454112No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18470.1752007884509765No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG18070.17140651041197322No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17430.16533566554956797No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16100.15271969106988206No Hit
ATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCT15090.14313913902139877No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT14570.13820657757069452No Hit
CAACCAAGACTACTCGTTAAGTAATGAATCCTCATTGGATGAGGAAGGGA14480.1373528650119188No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA14310.13574029684534236No Hit
CAAAAGGATTGGCCGATGATTACTTTTGGAAAAAGAAAGAAAAGCTGGGA13990.13270487441413975No Hit
CATGTTAACTTTTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG13910.1319460188063391No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA13900.13185116185536402No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA13070.12397803492443221No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC12640.11989918603250369No Hit
CATTTCAACTTCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG12000.11382834117009843No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT11470.10880092276841907No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC11320.10737806850379286No Hit
GTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTGATTGA11190.1061449281411168No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA11180.1060500711901417No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC10890.10329921961186432No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC10870.10310950570991417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC21450.0122.388421
GCACGCA21950.0119.57782
CACGCAC22750.0115.674213
CGCACTT25500.0104.587315
ACGCACT25700.0102.663124
GTAACAA8100.090.13454
AAACACG2100.089.484315
GCACTTT24100.086.9667366
GCACTTC9400.082.2674566
TAGTAAC9700.076.033842
GAAGCGG3300.072.320421
CGAACTT300.001959702572.275795
ACTTCGG702.8849172E-972.268918
GAAACAC2850.071.017914
GTGCGCT554.133615E-665.7146154
TAACAAG11400.064.0338065
CACTTGA4200.060.22417
ACAAGAG12400.058.86427
CAACGCA4500.054.6031889
GTATACG400.006110628654.2403221
Produced by FastQC (version 0.11.5)