FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143990
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT28030.2450196242974152No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT27230.23802655617619034No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT23640.2066451629821939No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC18580.16241400711544682No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT16550.1446690967578388No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT16330.14274600302450197No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA16180.1414348027517723No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC15670.13697672182449147No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT15650.13680189512146085No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC15540.13584034825479244No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC13870.12124231855173559No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT13430.11739613108506194No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA12920.11293805015778109No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12900.11276322345475048No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC12770.11162684988505144No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC12710.11110236977595958No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC12710.11110236977595958No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA12490.10917927604262274No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG12430.10865479593353089No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC12240.10699394225473999No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT12170.10638204879413282No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG11750.10271068803048979No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT11730.10253586132745916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA3700.0121.1151352
CACGCAC3900.0114.899063
AGCACGC4050.0112.462571
CGCACTT4850.095.3693165
ACGCACT4850.092.389024
TAGAAAC3100.079.273062
AACACGA1950.077.824636
AAACACG2800.077.434365
GAGACGC300.001932389172.53234145
GAAGCGG1400.072.297371
ACACGAG2100.072.2625667
GAAACAC3150.071.1248864
GTAGTAA11350.064.9720841
ACGTGCA902.8921932E-1064.247452
CGGTGCC350.003610777261.9501953
TAGTAAC11550.060.701322
CACGTGC2300.059.7239151
CCGCATT502.0419002E-457.8176464
CAACGCA1251.8189894E-1257.8100559
GCACTTT8900.056.838226
Produced by FastQC (version 0.11.5)