FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT47250.4156363722729635No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23070.2029361081129581No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA22980.20214441978481912No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC19070.16774996019567018No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG18630.1638794839247685No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA17520.1541153278777211No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17420.1532356741797889No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17330.15244398585164992No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT16760.14742995977343637No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA16450.1447030333098466No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT14730.12957298970541278No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA14270.12552658269492467No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG14160.12455896362719925No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13760.12104034883547043No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13650.12007272976774504No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13600.11963290291877894No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT13280.1168180110853959No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13260.11664208034580946No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12950.11391515388221964No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG12800.11259567333532135No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT12560.11048450446028407No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG12400.10907705854359256No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA12200.10731775114772815No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA11660.10256762117889429No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG11590.10195186359034175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1750.0111.556492
CGCACTT1700.0110.530755
GTAACAA13350.0109.8987054
CACGCAC1800.0108.4242553
GAAACAC5350.0104.01954
AAACACG5450.0100.7803345
TAGAAAC5900.095.5898362
AGCACGC2100.092.976031
ACGCACT2150.087.400254
AACACGT2150.087.38876
TAACAAG18050.082.485
GAAGCGG1300.077.877361
ACACGTG2450.076.688057
TAGTAAC20100.075.182952
ACGTGCA4000.074.112762
AACACGA5200.070.874056
CACGTGC5500.069.6850741
CGGAGCA1350.069.627172
ACACGAG5900.062.4652637
AGTAACA25050.061.750613
Produced by FastQC (version 0.11.5)