FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148097
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26660.23221034459631895No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT21580.18796321216761303No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT21010.18299847486754167No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20020.17437551008320726No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19740.1719366917603652No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC19400.16897526951119984No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG18600.1620072171602225No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT17410.15164223928814377No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG16300.14197406665116274No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG16180.14092885879851616No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA15050.1310864848527607No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC14920.1299541763457269No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC14350.12498943904565554No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT14090.12272482203158792No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC13480.11741168211396773No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13450.11715038015080609No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT13200.11497286379112566No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT13190.11488576313673844No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT12720.11079203238053928No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC12330.10739510685943783No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT12230.10652410031556567No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT11930.10391108068394916No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA11870.10338847675762587No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC11790.10269167152252814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC1250.0115.659863
GTAACAA11100.0115.259064
GAAACAC5050.0101.622954
TAGAAAC5450.095.5239642
AAACACG5300.095.4656145
GCACGCA1550.093.29852
TAGTAAC14400.090.382922
AGCACGC1700.089.335181
CGCACTT1650.087.6134645
ACGCACT1800.080.312344
AACACGT2800.080.025516
ACAAGAG19000.066.955137
ACACGTG3400.065.9033667
AGTAACA20150.065.2918553
CATAAGG902.8921932E-1064.2835161
TAACAAG20450.062.914615
AACACGA4850.061.103626
CCAAGCT4450.060.0988964
ACCAAGC4750.059.351773
ACACGAG5100.058.1083457
Produced by FastQC (version 0.11.5)