FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226855
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT37800.3081048697686361No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT33430.27248533852818796No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT26910.21934132395433856No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23760.19366591814028553No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA22090.1800538775975971No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT21740.177201054729369No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT20850.16994673372158894No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT20670.16847956767507163No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT19820.16155128356651763No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG19420.15829091457425695No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC18560.15128112124089643No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA17900.14590151240366628No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC17680.14410830945792288No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC17150.13978832054317747No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC16680.13595738697727117No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT16520.13465323938036688No Hit
AACCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATG15920.12976268589197584No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG15550.12674684457413468No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG15190.12381251248110006No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC14780.12047063426403283No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA14730.12006308814000025No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA14600.1190034682175155No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC14520.11835139441906338No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT14170.11549857155083527No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC14160.11541706232602875No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT13940.11362385938028535No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC13830.11272725790741367No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA13540.11036349038802468No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG13400.10922236124073342No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT13280.10824425054305521No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC13060.10645104759731182No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG13000.10596199224847272No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC12830.10457633542676192No Hit
AGCTTTAGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTG12810.10441331697714888No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT12700.10351671550427721No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC12660.10319067860505113No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC12600.10270162325621202No Hit
GCTTTATGGTCTGGCGGTTAAAGGACAATCTCATCTGAGAGGAGATACTG12520.10204954945775989No Hit
AATATACTGTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAA12400.10107143876008166No Hit
TCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAGATTTCTTTCT12380.10090842031046865No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT12320.10041936496162952No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAC100.0071561406144.557543
GCACGCA5350.0120.263992
AGCACGC5450.0118.062131
CACGCAC5600.0114.871623
CGCACTT6000.0108.396055
AAACACG3750.0107.914285
ACGCACT6150.0104.594294
GAAACAC4200.096.3677754
TAGAAAC5200.084.805862
ACACGTG2100.079.14317
AACACGT2200.075.545686
GCACTTT11200.074.196656
GTAGTAA14000.072.812881
CAACGCA702.8885552E-972.2610869
TAGTAAC14250.068.9959262
AACACGA3550.067.172276
GTACTAT3150.064.232086
ACAACGC808.301868E-963.2284478
ACACGAG3800.062.7530487
CGGTGCC350.003607220261.9658782
Produced by FastQC (version 0.11.5)