FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835842
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT28220.33762361786079187No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT17300.20697691668999643No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT16480.1971664501185631No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT13630.1630690967910203No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13540.16199233826488738No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT12690.15182295218474307No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA12680.15170331234850606No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC12330.14751591808021133No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA11150.13339841740424627No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC11030.13196273936940234No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC10010.11975947607322916No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT9830.11760595902096328No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC9460.11317928508019458No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC9430.11282036557148362No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA9250.11066684851921775No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC9240.11054720868298076No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT9170.1097097298293218No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA9140.10935081032061084No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC9130.10923117048437384No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC9070.10851333146695188No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT8590.10277061932757625No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA100.0071619186144.51581
AGCACGC4750.0118.6550751
CACGCAC4950.0113.8472753
GCACGCA5000.0112.715562
ACGCACT5950.095.916074
CGCACTT5900.095.498785
AAGCGGT406.2418076E-790.3169562
AAACACG3150.084.848935
GAAACAC3450.077.47544
GTAGTAA11250.077.075091
TAGTAAC10700.076.980442
AAGCACG903.6379788E-1272.25791
GCACTTT8650.069.309256
GAAGCGG1050.068.817041
ACAACGC1050.068.792318
GTAACAA12400.066.9973144
TAGAAAC4400.064.042942
AGTAACA13350.061.6959763
TAACAAG13800.059.6734245
ACGTGCA1007.403287E-1057.802852
Produced by FastQC (version 0.11.5)