FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031087
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC71730.6956735949536751No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA48090.4664009923507909No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG37580.36446972951845963No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT37240.36117223861807973No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT28670.27805607092321016No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA25590.24818468276682762No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT25400.24634196726367416No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG25360.2459540271577471No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG23960.23237612345030054No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT22730.22044696519304385No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT22440.21763439942507276No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC20980.2034745855587356No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT20840.20211679518799094No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT19080.1850474305272009No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA18930.1835926551299745No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT18100.1755428979319883No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA17270.1674931407340021No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA16690.1618680091980599No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA16110.15624287766211775No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT14900.1445076894578246No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA14710.14266497395467112No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC14190.13762175257761955No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC14030.13606999215391136No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA13790.1337423515183491No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC13770.13354838146538556No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG13530.13122074082982327No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG13490.13083280072389625No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG13460.13054184564445095No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA13380.12976596543259686No Hit
GTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAG13040.12646847453221696No Hit
AAGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA12640.12258907347294651No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT12510.12132826812868362No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT12320.11948555262553014No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC12240.11870967241367604No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA11950.11589710664570496No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC11930.11570313659274145No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA11780.11424836119551503No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT11660.1130845408777339No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC11490.11143579542754393No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC11460.11114484034809866No Hit
AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAA11450.11104785532161689No Hit
ATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGTCCATTGT11110.10775036442123702No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT10810.10484081362678417No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG10640.10319206817659421No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA10410.1009614125675137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC21950.0126.182281
GCACGCA22350.0124.5711062
ACGCACT23100.0122.0622254
CGCACTT23700.0121.400715
CACGCAC24750.0112.756293
GCACTTC14950.094.772056
GAAGCGG7650.093.585351
CACTTGA14650.089.8047267
TAGTAAC7250.088.774072
GCGGTGC8050.088.036811
GCACTTG16900.086.4032446
GTAGTAA7950.081.867121
CTTCGGT1400.077.451299
ACTTCGG2300.075.430818
TAACAAG9050.070.294375
CTTGATT19550.066.926369
CACTTCG2750.063.0875937
AGTAACA10200.063.0838973
ACTTCAG15050.060.0397578
ACTTGAT23100.056.3282058
Produced by FastQC (version 0.11.5)