FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214592
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT40040.3296580250816735No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30630.25218344925703445No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT26070.21463997786911163No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC25350.2087120613341764No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA24840.20451312045526399No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT19250.15848943513541996No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA16390.13494243334387185No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15780.12992017072399623No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT15540.12794419854568448No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT14940.12300426809990517No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA14120.11625302982400673No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC14090.11600603330171777No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT13840.11394772894930973No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT13660.11246574981557593No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG13460.11081910633364948No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA13290.10941945937401201No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC12960.10670249762883338No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA12650.10415020023184741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA6450.0115.4648362
CACGCAC6450.0115.44583
AGCACGC6700.0112.254161
CGCACTT7800.0101.9316945
ACGCACT7700.097.635434
GAAACAC4300.094.142224
AAACACG4300.094.130585
TAGTAAC18150.084.0578162
AACACGT2500.083.836556
TAGAAAC5100.083.647722
GTAGTAA20950.075.251711
GTAACAA20750.073.1587144
ACACGTG2900.072.272897
AGTAACA22150.069.519483
GCACTTT14700.064.406446
ACGTGCA1600.063.267452
GCCCGCA606.915656E-660.2547152
CCCGCAC606.9224207E-660.2447783
AACACGA3400.059.5556531
TAACAAG25650.059.4574475
Produced by FastQC (version 0.11.5)