FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_FLUB12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_FLUB12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218176
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT39410.3235164705264264No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26360.21638909320163918No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20360.16713512661553012No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA19930.1636052590101923No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19870.1631127193443312No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17030.13979917516023957No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT16410.1347095986130083No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA16250.1333961595040454No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC16240.1333140695597352No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG15550.12764986340233267No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA14920.1224781969107912No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA14330.1176348901964905No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA14050.11533637175580538No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG13770.11303785331512031No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT13290.10909753598823159No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13230.1086049963223705No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG13110.10761991699064831No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG12670.10400795944100032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA13100.0122.4941254
GAAACAC5000.0102.641244
AAACACG5550.093.7681355
GCACGCA1350.091.04122
CACGCAC1350.091.0299763
TAGAAAC5900.090.678122
AGCACGC1400.087.804161
TAGTAAC20350.079.580462
AACACGT2100.079.153626
TAACAAG21000.076.754045
CGCACTT1800.076.295135
ACGTGCA3250.073.409692
ACACGTG2400.069.262267
GAGCATC2050.063.457196
CACGTGC4800.063.270651
AGTAACA25850.063.200013
ACGCACT2100.061.9564864
ACAAGAG26200.061.239517
AACACGA5850.059.2990236
GAAGCGG1154.0017767E-1156.5898971
Produced by FastQC (version 0.11.5)