FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_DARPA_AHA5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_DARPA_AHA5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961442
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC14190.147590806309689No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT10480.10900293517445671No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA9810.102034236074563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.092.558861
AGCGAAA2450.070.835851
GCAAAAG17700.048.6010863
CGAAAGC3650.047.53253
GAGCAAA20950.043.1453481
CAAAAGC23800.036.13514
TGACCGA2103.9108272E-1034.412596
CCGAGCC2455.820766E-1132.4461569
ATGACCG2452.040906E-929.4965045
AAGCAGG33550.028.4326937
ACAAGGT7250.027.9097968
AGCAAAA32100.027.4786222
GTATTAT3505.456968E-1226.8585971
GTATATA6600.026.295131
GTACTGA10550.026.0296176
GAGCGGT4750.025.8637778
CTAGATC1400.001016571225.8175053
GTATAGG7700.025.3560161
TTAGGCG2901.2314558E-824.9207574
AAAAGCA35900.024.558515
Produced by FastQC (version 0.11.5)