FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_B2S3_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_B2S3_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981723
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG11750.11968752896692855No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA11110.11316837845298522No Hit
ATGTTATGCAGTGCAGTTTTGGTGTAAAATAAACATTGAGGTATAAATCA10660.1085846007478688No Hit
GAGCAAAAGCAGGGCGGCTGGGGGGAGGCGCAGAGACTGAGCTGCTCAGT10440.10634364275870077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA24400.062.8276371
GCAAAAG24350.062.64693
CAAAAGC25300.060.282254
GCGAAAG5400.054.9028322
GAGCGAA3500.053.7167781
AGCGAAA5700.052.0132141
CGAAAGC5650.051.1832243
AGCAAAA30100.050.9300462
AAGCAGG47600.036.5926067
AAAGCAG51300.034.235146
AAAAGCA47100.033.4534575
AGCAGGC19250.032.288738
TATGCCG900.003682205232.1251374
GCAGGCC10900.031.8271669
CAGTACG1402.8057824E-530.9746519
ATATACG950.00478797430.4467581
ATACGGC1204.1254325E-430.1234573
AACAAGG16700.029.8770222
GCCCTAT4800.027.1166421
TATACGG1100.00979528826.2949282
Produced by FastQC (version 0.11.5)