FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_B2S19_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_B2S19_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064469
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC46980.44134681235432877No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG44130.41457289972746975No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT40080.3765257607314069No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA40060.37633787362525356No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA32850.308604571856954No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT29570.277791086447797No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT27830.2614449082124515No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTATGGGGAG26390.2479170365694069No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT26090.24509872997710594No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA26070.24491084287095255No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA22950.2156004543110227No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG22610.21240637350641495No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA18930.17783514597418995No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA17640.16571642762729585No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT16950.15923432246500369No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA16670.15660390297885612No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC16630.15622812876654932No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG16310.15322193506809498No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA16140.1516248946657911No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT15850.14890053162656686No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC15360.14429729752580864No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT14960.1405395554027407No Hit
ATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTATGGGGAG14590.13706364393890288No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13260.12456915137970198No Hit
GAATATAATCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAA13070.12278422387124471No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT12910.12128112702201754No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT12880.12099929636278746No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG12650.1188385946420234No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA12160.11423536054126517No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT12110.11376564277588168No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA12000.112732263692038No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT11920.11198071526742441No Hit
TCTCAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACT11900.11179282816127102No Hit
TTCCAAGTGAGAACATGAAATAAAGGGCTCTCTTATGACAAAAACGTCTC11800.11085339263050403No Hit
CTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCT11800.11085339263050403No Hit
GAAAAAAGGTCCTGCATTCCAAGTGAGAACATGAAATAAAGGGCTCTCTT11690.10982001354666035No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC11660.10953818288743027No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA11650.10944423933435356No Hit
ATATAATGCAGAATTGTTAGTTCTACTGGAAAATGAAAGGACTCTGGATT11590.10888057801589338No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA11570.10869269090973997No Hit
GTAGGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAA11360.10671987629512932No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG11330.10643804563589922No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA11310.10625015852974583No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC11100.10427734391513514No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT11020.10352579549052156No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG10990.10324396483129146No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG10960.10296213417206138No Hit
ATACTGGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGG10830.10174086798206429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2600.0125.184271
GCAAAAG22250.096.844333
AGCAGGC7000.093.960978
CAAAAGC28100.076.411094
TTAGTAG801.0186341E-1072.328691
AAGCAGG34350.071.5412147
AGCGAAA4950.065.744072
AACGTAT554.1365E-665.706979
GCAGGCA10400.063.2429589
GCAGGGG10100.062.2589849
TAAACTA3800.060.8711245
AGTAGAA10700.059.485281
GAGCAAA37200.058.9128841
CTATTCG3800.057.0613179
GGATACG400.00604444654.389862145
TGGGCCC1405.456968E-1251.79987145
GGACAGT17650.051.1929636
AAAGCAG47900.051.157486
AAAAGCA42450.050.7438665
ACAGACT3350.049.623478
Produced by FastQC (version 0.11.5)