FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_B2S14_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_B2S14_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314231
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATGCAGTGCAGTTTTGGTGTAAAATAAACATTGAGGTATAAATCA4070.12952254869825064No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA3380.10756418049142191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA6400.072.284521
TTGAACG300.001958714872.273027
GCAAAAG6800.065.895993
GTGATCG350.003608774961.9483039
AGCAAAA7700.059.141892
GACGGCT400.00612251354.204767
AGCGAAA1500.053.008661
GCGAAAG1600.049.695612
CAAAAGC9400.048.43834
ACGGCTC450.00975309248.1820148
CGAAAGC1650.048.182013
ACAACGG803.7984566E-545.1706358
CTATACT1704.0017767E-1142.513544
GAGCGAA1401.4466423E-841.3054471
AAGCAGG12800.040.088947
CGTCAGG951.0454406E-438.044491
AGCAGGC5400.036.136518
AAAGCAG14100.035.8802226
CAACGGC1051.8852466E-434.415729
CCAGAAC2009.1858965E-932.5228583
Produced by FastQC (version 0.11.5)