FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_B1S4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_B1S4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852932
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA8560.10035970042160454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA19300.065.5557861
GAGCGAA3650.059.423561
GCAAAAG21200.057.9651343
AGCAAAA22550.055.4566152
GCGAAAG5350.052.6944162
AGCGAAA5500.051.2663271
CAAAAGC24750.049.3530854
CGAAAGC5750.049.028723
AGCAGGC15350.042.8434838
AAGCAGG41400.035.0871057
GCAGGCC8550.033.810049
TAATACG1501.161201E-633.7294774
AAAGCAG43950.033.0493936
TATAGCG1352.1890875E-532.1195375
AAAAGCA42500.029.7578095
GTTATGC4750.028.9143753
TACGTTG1658.601857E-526.2796217
TAGCGGC1450.001249323224.920337
GCACCGT1500.00152212124.0896557
GCCCTAT4550.023.8347241
Produced by FastQC (version 0.11.5)