FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_B1S10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_B1S10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110144
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG114021.0270739651792922No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA111241.0020321687997233No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA79290.7142316672431684No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA52160.4698489565317652No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA51830.46687636919174447No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT46530.4191348149429263No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC36590.3295968811253315No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA31860.2869897959183674No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG31800.286449325492909No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT30580.27545976017525653No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT29000.26122737230485416No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT27830.25068819900841693No Hit
GTAAAGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT27650.24906678773204197No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG27380.24663467081747953No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG25790.23231220454283408No Hit
GTGCCTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC24620.22177303124639686No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG22740.20483829124870287No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC22470.20240617433414043No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG22440.20213593912141126No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC21470.19339833390983513No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC21280.1916868442292171No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC19490.1755628098697106No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG19440.17511241784849532No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG18550.16709543987086362No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG18540.16700536146662054No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT18530.1669152830623775No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA18390.16565418540297475No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT18290.16475340136054423No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC18070.16277167646719704No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT17980.16196097082900957No Hit
GTAAGGCACCTTGGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAACAT17860.16088002997809292No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC17790.16024948114839155No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC17370.15646618817018332No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA17290.1557455609362389No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA16750.15088132710711402No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA16560.14916983742649603No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA15350.1382703505130866No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG15310.1379100368961144No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA14950.13466721434336446No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG14810.13340611668396174No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA14780.13313588147123256No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG14430.1299831373227257No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA14090.1269204715784619No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT13950.12565937391905915No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT13850.12475858987662863No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG13520.12178600253660787No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13310.11989435604750376No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT13220.11908365040931626No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA13110.11809278796264268No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT13050.11755231753718437No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA12960.11674161189899689No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA12950.11665153349475384No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT12860.11584082785656635No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC12640.11385910296321919No Hit
GTGCTTTACTGAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC12600.11349878934624696No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG12360.1113369076444137No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT12330.11106667243168454No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC12110.10908494753833736No Hit
GGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTGGATCAATCTGT11930.10746353626196242No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG11770.10602228179407355No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA11410.10277945924132366No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA11290.10169851839040701No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA11180.10070765594373343No Hit
GTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATC11110.10007710711403206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3000.0122.943741
GTAATTT4550.094.03619145
GCAAAAG34500.088.8640063
AGCGAAA7300.084.208031
GGGCTTA353.4328717E-582.87935145
CAAAAGC41250.075.0136264
AGCAGGC3150.073.437968
CGGCTAA300.001957798872.293755
AGCAAAA42750.072.0563352
TAAACTA27100.070.1596155
GCAGGTC28150.068.0532159
TTTAAAC27700.067.8691943
GCAGGGT3000.067.471129
AAGCAGG51850.066.504467
GAGCAAA47250.065.814891
CTATTCG29250.064.999659
TTTTTAA29000.064.838481
AACTGCG451.19233155E-464.461716145
CAATACG451.2087049E-464.28431
TTTTAAA29300.063.919132
Produced by FastQC (version 0.11.5)