FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17815959
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11282416.332754807080551No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA218870.12285052968521089No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG214200.12022928431750432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG77050.058.6073539
CGCACTT28850.056.4061435
GCAAAAG256600.056.324813
ACGCACT28650.055.8373534
GAGCAAA290450.050.3842121
GAGCGAA36600.049.0599561
CACGCAC34550.045.5042463
CAAAAGC322250.045.3632744
GCAGGTC105500.045.089989
TAAACTA104850.044.3910685
TTTAAAC114550.041.2344863
AGCAAAA364250.040.020862
GCACGCA42100.036.8537252
AAAGCAG459600.036.1272936
AAGCAGG478000.034.4135637
TAGTAAC114150.032.983572
GCAGGTA116350.031.8193539
AAAAGCA465250.031.7907775
TAACAAG123350.030.9692615
AGCAGGG171800.030.417928
Produced by FastQC (version 0.11.5)