FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084360
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA45710.42153897229702314No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATTCGAATTCTTTTGGTCG45400.4186801431258992No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA33260.30672470397285034No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC27710.2555424397801468No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA26780.2469659522667749No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG25120.23165738315688517No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATG24100.22225091298092883No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT23830.21976096499317568No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATTC22600.2084178686045225No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20070.18508613375631708No Hit
ATTCGACACTAATTGATGGCCATTCGAATTCTTTTGGTCGCTGTCTGGCT18950.17475746062193367No Hit
ATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCC18440.1700542255339555No Hit
GGATTGGGTGATGCCCCATTCCTCGATCGGCTACGCCGAGATCAAAAGTC18320.168947581983843No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18160.16747205725035966No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG17850.16461322807923567No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG17090.15760448559518977No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA17030.15705116382013354No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC16410.15133350547788557No Hit
CTATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAAGA16080.14829023571507619No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15960.14718359216496368No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT15910.14672249068575013No Hit
CCATATGGCCATCATCAAAAAGTACACATCGGGAAGGCAAGAGAAGAACC15450.1424803570769855No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT15220.1403592902726032No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA15090.139160426426648No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAA14820.13667047843889485No Hit
GAGGAGGGAACAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG14550.1341805304511417No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14030.1293850750673208No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA13680.12615736471282601No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATTCGAAT13650.12588070382529787No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG13640.12578848352945515No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGT13570.1251429414585562No Hit
CAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCA13270.12237633258327493No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTGGACACAGTAC13260.12228411228743222No Hit
TTTTTGGACAATATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13050.12034748607473533No Hit
CACTATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAA12980.11970194400383637No Hit
TTCTTAATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGGGCCCT12930.11924084252462283No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12530.11555203069091445No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGA12510.11536759009922903No Hit
ACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGGGAAGGCAAGA12400.11435316684495925No Hit
AATTGATGGCCATTCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT12050.11112545649046442No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAG12010.1107565753070936No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11860.10937327086945295No Hit
GAATGAAGTGGATGATGGCAATGAAATACCCAATTACGGCAGACAAGAGA11770.10854328820686858No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATT11750.10835884761518313No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAACAATAGTTGGAGAAATTTCACC11690.1078055258401269No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAAATGAGGAATC11670.10762108524844148No Hit
ATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAAGATT11640.10734442436091335No Hit
ACTAAGGGCTTTCACTGAGGAGGGAACAATAGTTGGAGAAATTTCACCAT11530.10633000110664353No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG11490.10596111992327271No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT11020.10162676601866538No Hit
GGATAGAGCAGTTAAACTATACAAGAAACTCAAAAGAGAAATAACGTTCC10970.10116566453945185No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT10940.10088900365192371No Hit
TTACCAACACTATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCT10880.10033568187686745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1350.0134.271351
TACATCG203.8661796E-4108.749756145
CCGCGCG1200.0102.7081145
AGCGAGC1200.090.624794145
ATGGGCG259.3870464E-486.9998145
GCGAAAG6200.079.519622
CGGACCC502.3064022E-672.49983145
GCAGGGT8200.069.8474049
CAGGTAC4700.064.790083
CAATTCG13200.063.711989
GTACTGA4900.063.6252376
CCGAACC350.003566616262.14272145
TTTAAAC13550.062.0691453
TACTCCT2700.061.761975
CATACTC2750.060.6390273
TAGGCCG606.8060726E-660.41653145
GTAGAAA11750.059.856451
CAAAAGC25100.058.0603184
AGCAGGG11150.056.5693788
GAGCAAA26700.054.5835651
Produced by FastQC (version 0.11.5)