FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316468
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG66560.5055952746287794No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC53750.408289453294725No Hit
GAGCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAA45700.3471409863361662No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT42610.323669090323502No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT42310.3213902654679035No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC38920.29563954459964087No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT38310.291005934059924No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA36460.27695318078373343No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC36240.27528204255629457No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG35640.27072439284509764No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT34430.26153313259418387No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC32570.24740441848947337No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA32020.24322657292087618No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA30400.23092091870064446No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA30100.228642093845046No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG29120.221197932650091No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT27890.21185475074213728No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG26200.19901737072226594No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT25770.19575105509590812No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT24530.18633191235943447No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC24230.18405308750383603No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT24140.1833694400471565No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23060.17516567056700202No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT23010.17478586642440225No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC22920.17410221896772274No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT22900.17395029731068282No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC22120.1680253526861268No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT21780.16544268451644856No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC21670.16460711540272913No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT21100.16027734817709202No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG20630.15670718923665444No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCACA20480.1555677768088552No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20290.1541245210669762No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20190.1533649127817767No Hit
GTCCCAATAGTTCTCATTGCATGCACCATCTGCCTAGTCTTATTAGCAAC20160.15313703029621684No Hit
TATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCCA19730.14987071466985905No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGA19530.14835149809946008No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCATCTTTT19510.14819957644242018No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT18780.1426544359604639No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC18770.14257847513194397No Hit
CTTTTCAGACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG18540.14083137607598514No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA18100.1374890996211074No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA17970.13650160885034804No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT17880.1358179613936685No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC17880.1358179613936685No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT17830.13543815725106878No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG17750.13483047062290918No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT17710.1345266273088294No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGT17660.13414682316622964No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC17270.13118435085395164No Hit
GTTCACAAGTGGCACAAACTAGACCGAAAGCAGCTTCTGTGGTCACTGTT17220.1308045467113519No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC16830.12784207439907389No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA16700.12685458362831453No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG16680.12670266197127464No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG16490.12525940622939563No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGTAGTAG16450.12495556291531583No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC16280.1236642288304767No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT16180.1229046205452772No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT15910.1208536781752386No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA15800.12001810906151916No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT15790.11994214823299922No Hit
GCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGATTGGACC15770.11979022657595932No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT15710.11933446160483961No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGA15490.11766332337740074No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCATCAGGC15400.1169796759207212No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG15230.11568834183588207No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA15130.11492873355068257No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT15080.11454892940808284No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATG15060.11439700775104295No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCAAGTTGCATGGGCCTCATATA15060.11439700775104295No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA14990.1138652819514033No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT14630.11113069212468514No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGTA14480.10999127969688592No Hit
GCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC14470.10991531886836597No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC14380.10923167141168642No Hit
GTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTAGTG14340.10892782809760664No Hit
TGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGATTGGAC14310.10869994561204677No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG14300.10862398478352683No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC14290.10854802395500687No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC14250.10824418064092708No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT14120.10725668987016776No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC14060.10680092489904805No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG14050.1067249640705281No Hit
GTCCAATCGCACGCAAAGAGGGCCTATCATCTTTTGCCTAGGCATAAGCA14020.10649708158496826No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT14020.10649708158496826No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGCACCATCTG13990.10626919909940842No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG13930.10581343412828872No Hit
CTTTCTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT13930.10581343412828872No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT13890.10550959081420894No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG13880.10543362998568899No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA13880.10543362998568899No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAA13790.10474998252900944No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT13760.1045221000434496No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13690.10399037424380995No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG13670.10383845258677006No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCAAGTTGC13640.10361057010121022No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC13280.10087598027449206No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCATCAGGCCC13180.10011637198929256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT100.007088027145.021134
GAGCGAA2750.0137.110871
CGAGGCG256.4658416E-6115.999275145
AGCGAGC2700.091.29572145
AGGCTCG651.6370905E-1189.2302145
AGTAGAA6300.088.6240161
CCGCACG353.437789E-582.85663145
GGAGGGC451.2331511E-680.555046145
GAGCAAA36650.078.742711
TAGTATA608.0339305E-872.49954145
AGCAGGG22350.070.066678
GTAGAAA12250.069.846911
CAAAAGC41450.069.7990654
GCAGGGT21250.068.576049
TTCTACT1100.065.9186864
GCAGGTA15250.065.130749
AAAAGCA45450.063.6561365
GCGAAAG8550.063.605762
TAGAAAC14350.062.151912
AGTAACC350.003564696862.151912
Produced by FastQC (version 0.11.5)