FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152206
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC42370.3677293817251429No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT39900.3462922428801794No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG38480.33396805779522065No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30700.26644540993537613No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC29730.25802677646184796No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC28550.24778555223631885No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG28150.2443139508039361No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT27950.24257815008774472No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA24720.21454496852125401No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC24270.21063941690982343No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23890.2073413955490598No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT22590.19605869089381586No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT22450.1948436303924819No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20070.17418760186980453No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG19940.17305933140428015No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA19590.17002168015094524No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA18980.16472748796656153No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT18560.16108230646255964No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC17530.15214293277417407No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT17470.15162219255931664No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT17350.15058071212960183No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17060.14806380109112433No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC16390.14224886869188325No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA16370.14207528862026408No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT16220.14077343808312054No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT16040.1392112174385483No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT15590.13530566582711773No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC15470.1342641853974029No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT15380.13348307507511678No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA15320.13296233486025938No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG15170.13166048432311583No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGA15060.13070579392921058No Hit
CATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCA14790.1283624629623522No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA14750.12801530281911394No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC14680.12740777256844696No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT14610.12680024231777998No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC14500.12584555192387473No Hit
TATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCCA14380.1248040714941599No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG14170.12298148074215895No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT13980.12133247006177715No Hit
CTTTTCAGACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG13680.11872876898749009No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA13670.11864197895168052No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG13560.11768728855777527No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC13170.11430247716120207No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT13160.1142156871253925No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCACA12920.11213272626596285No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC12850.11152519601529587No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC12820.11126482590786717No Hit
GTCCCAATAGTTCTCATTGCATGCACCATCTGCCTAGTCTTATTAGCAAC12760.11074408569300975No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT12730.11048371558558105No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA12570.10909507501262795No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT12550.1089214949410088No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC12420.10779322447548442No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG12270.10649137393834089No Hit
TTTTCAACCTGTGTCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT12130.10527631343700693No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGTCTGATG12030.10440841307891124No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC12020.10432162304310168No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG12000.10414804297148253No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT11970.10388767286405383No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT11970.10388767286405383No Hit
GCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGATTGGACC11800.10241224225529116No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG11780.10223866218367203No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT11750.10197829207624332No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC11750.10197829207624332No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCATCTTTT11690.10145755186138591No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG11670.10128397178976675No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC11670.10128397178976675No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC11650.10111039171814762No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA11570.10041607143167107No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGTA11560.10032928139586149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAA151.2300565E-4144.99991145
CCGCACG256.4653123E-6115.99994145
CAGGTAC1700.0110.882293
GAGCGAA900.0104.731251
AGTAGAA5350.090.802221
GTACTGA2150.087.674376
GCGAAAG4800.087.604112
CGAGGCG651.5643309E-978.07687145
CGGACCC300.001935821872.499954145
AGCGAGC3050.068.93439145
AGGTACT2800.067.3213964
GTAGAAA9950.066.312251
TATGACC3300.054.924214
GCCCACC805.874517E-754.374966145
TGGCGGT400.00605113454.374966145
CTACACT4450.052.1348044
TAGAAAC12800.051.542942
GCAGGTA4400.051.0795142
CAATTCG3700.048.986469
ACCGAGC3750.048.333318
Produced by FastQC (version 0.11.5)