FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186869
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC27800.23422972543726395No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA24390.20549866918758516No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA21210.17870548476706358No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20880.17592505996870758No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT20710.17449271992106963No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT18270.15393442747261915No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC18260.15385017217569927No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC18150.15292336390958058No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18030.15191230034654202No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG17440.14694123782826918No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA17090.1439923024360734No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG16930.14264421768535532No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT16710.14079060115311798No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAAATGAGGAATC16550.13944251640239994No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAA16520.1391897505116403No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCACA16520.1391897505116403No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC15800.133123369133409No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG15750.1327020926488096No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA15020.12655145597365844No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC14890.1254561371137No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT14330.12073784048618676No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG13850.11669358623403257No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC13340.11239656609111873No Hit
CCTTAACCCTGATCCTCTCACATTCACAGTCAATGAAGAAAATTGCATCC13230.11146975782500006No Hit
CTGTAGATACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT13170.11096422604348079No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT12780.1076782694636055No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA12460.10498209996216937No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA12380.10430805758681035No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATG12370.10422380228989046No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG12330.10388678110221095No Hit
GAGCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAA12100.10194890927305372No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT12020.10127486689769469No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT12000.10110635630385494No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT11950.10068507981925554No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT11940.10060082452233567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1300.0122.70241
CCGCACG1000.094.24981145
CGGACCC554.395224E-879.09075145
AGCGAGC851.8189894E-1276.76455145
GGGGTAT300.001935200572.505961
GGATTAA406.663767E-572.499855145
GTATAAA300.001935847672.49985145
GCGAAAG4550.070.11272
GCAGGGT7300.068.527259
AGCAGGG8700.068.33328
ACAGCCT606.8064583E-660.416542145
GTAGAAA7450.059.36731
GTACTGA3850.058.3789636
CAGGTAC3900.057.6305163
AGTAGAA7550.054.7396051
CAAAAGC20000.054.377184
CAATTCG5100.054.0194979
TAGGCCG553.2192798E-452.727165145
TTATACT4550.052.5845264
GCAGGTA6100.052.2949759
Produced by FastQC (version 0.11.5)