FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050071
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG29350.2795049096680129No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA26220.24969740141380917No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC22390.21322367725610936No Hit
GAGCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAA18210.1734168451466615No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC17380.16551261771823048No Hit
GTATCTTGGGTAGTCATAAGTCCCATTCTTGACACTTTCCATGCATGTGT16430.1564656104206287No Hit
CTGTAGATACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT15800.15046601610748225No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT15600.14856138299219765No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT15480.14741860312302693No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT14810.14103808218682354No Hit
GTCTATGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAA13740.13084829502005102No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC13530.12884843024900222No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC13360.1272294921010103No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC13180.12551532229725418No Hit
GTCCTACATTGTGGAAACGTCTAATTCAGACAATGGAACATGTTACCCAG12650.12046804454175003No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT12390.11799202149188007No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG12300.117134936590002No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11850.11284951208061168No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA11560.11008779406344903No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA11410.10865931922698562No Hit
GCTTTACTCCATCTATTTTTTCTCTGCTTAGTTTTGCTTCCTCTGAGTAT11320.10780223432510755No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT11310.10770700266934331No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT11290.10751653935781484No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT11050.10523097961947336No Hit
ATGTAGGACCATGATCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC11040.10513574796370913No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT11000.1047548213406522No Hit
ATGGAAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACAAG10980.10456435802912376No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT10820.10304065153689608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG151.2297991E-4145.006621
TACGTGG100.0070899455145.00663
GTACGGA100.0070899455145.00662
GAGCGAA1300.0128.275091
AGCGAGC800.0108.74978145
GCTATCC259.385177E-487.0039753
GTGTGCC259.386953E-486.99983145
GTAGAAA4100.076.0400541
AGCAGGG13400.075.7461248
GCAGGGT10700.073.177439
TTCGCTC300.001935418972.503314
CGCAAAA300.001935418972.503312
GTGGGCC300.001935784672.499855145
TCTATGA2450.071.023652
CAATACG1850.070.5437551
GCGAAAG3450.069.3512
GCAGGTA5200.066.922949
GGGGTTA350.00356590862.1456951
TACGGAT2150.060.7004474
CGGATAG2150.060.7004476
Produced by FastQC (version 0.11.5)