FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Mirella1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Mirella1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130545
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC37190.32895638829060325No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG36800.32550672463280983No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT36340.3214378905748997No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT26770.2367884515875087No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC24530.21697499878377244No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT24070.21290616472586232No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG23850.21096020061120965No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC23230.20547611992446121No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA21930.19397724106515No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT20630.1824783622058388No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19260.1703603129464108No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT19070.16867970757466533No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18350.16231109774489294No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA18200.16098430403035704No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA17890.15824226368698283No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG16890.14939697225674342No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA16720.14789327271360272No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC15620.13816345214033937No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT15230.13471378848254603No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT14520.12843363156707607No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC14140.12507242082358508No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT14090.12463015625207312No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA13760.12171121008009411No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT13620.1204728692798606No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT13570.12003060470834864No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG13500.11941143430823187No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC13340.11799618767939356No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG13160.11640403522195047No Hit
TATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCCA13150.11631558230764807No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGA12850.11366199487857626No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT12760.11286591864985472No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC12690.11224674824973796No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT12680.11215829533543556No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA12660.11198138950683077No Hit
CAATATGGGTTAGCCACTCAATTCAAATTGGAAATCAAAGCCAGATTGAA12540.11091995453520204No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT12280.10862017876333982No Hit
CTTTTCAGACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG12230.10817791419182783No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC12180.10773564962031587No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC12130.1072933850488039No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC12060.10667421464868714No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT11680.10331300390519617No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT11600.102605380590777No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG11460.10136703979054348No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC11450.1012785868762411No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG11350.10039405773321716No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA11330.10021715190461239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA404.811227E-9108.768441
GCGGTAG259.38734E-486.99936145
GCGAAAG2850.083.961612
AGCGAGC1250.075.39945145
AAACTCG300.00193586472.49947145
CCTCGCG651.3982935E-766.92259145
TGACCGG502.0104297E-457.9995739
GGTAAGC400.00604703154.384222
GTAGAAA8300.052.418531
CCTTATA1850.050.954592
CAATTCG1750.049.7139179
CCAACAC15750.046.4999473
CCATGTC14850.046.380139
AGTAGAA7500.043.507381
TACTCTA5500.043.4996837
GGTCAGA1703.8198777E-1142.6542931
ATCCAAC16850.041.7429851
GAGTTTA700.001056737641.428277
GCGGCCC700.001056737641.42827145
AAGGTAG2850.040.7086565
Produced by FastQC (version 0.11.5)