FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_Lib_prep_NC_water.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_Lib_prep_NC_water.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATCAGAAGACAGTGAAACAATATCTTCAAGTATAGGATCAGAAAAAA12.941176470588235No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG12.941176470588235No Hit
CATACAAGCTGTTGAATACCGACTTTGCTAATAAAGTCCTGCAGACCTTC12.941176470588235No Hit
CTTTAGTTCCATTTGGCATTTGCTATATTTAATATGTTAGAGGTATAAAA12.941176470588235No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC12.941176470588235No Hit
GGGGCGGGAATACCGCTATATGATGCAATCAAATGCATGAGAACATTCTT12.941176470588235No Hit
GTTCTAAGGATAACGAGGAGAAGGATTCCTTTTAAAAGAAGTAATCACAA12.941176470588235No Hit
GCACTATATCTTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGC12.941176470588235No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA12.941176470588235No Hit
GGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGG12.941176470588235No Hit
GTCTTATAGGGAGGAGCTTCTTTTTCGCCATTTTCTTTCTCTCTCTATTT12.941176470588235No Hit
GGGGTTTTTATCAGTGTCACCCATGAACTCGAGGTGCAATTTTGATGGGC12.941176470588235No Hit
TTGGAGTCCATTTGATTGTTCCAATCGATTCCATTCGATTACTTTCGAAG12.941176470588235No Hit
CCCCTGGAAAGACACATCTTCTGGTCTTGCACTTTCCATCATCCTTATGA12.941176470588235No Hit
CAATTTAACTATCAATATAAAAAACAAATTAATAACAATGCACATTTTAA12.941176470588235No Hit
CAGCAAAGACAATATAATAAGAACACATACCAAAACATAAGTGTGTTCAA12.941176470588235No Hit
GAACTCTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTATT12.941176470588235No Hit
ATTCAAGGCTCTGCTATTTTGACCAATCATAGAAGAACAAGTTTAAATCC12.941176470588235No Hit
GTAAAGAAAATGAAGAAAAACTTCCAGCAAAAAACCAGCAACAGAATAAA12.941176470588235No Hit
ACCCAGAAGCACGGCCTCATACAGTCTAGCCCTGTTAGCTAAGGATGTTG12.941176470588235No Hit
GTATGGGCCTCATATACAACAGAATGGGGGCTGTGACCACTGAAGTGGCA12.941176470588235No Hit
CTTTATTTGTTTTCAAAATAATGAATTAGCATCTTGGTATCTTACAAAAT12.941176470588235No Hit
AAAGACCACGGCATCAGACCGGACACCAGAACAAGTTGGCGGCAGAAAAT12.941176470588235No Hit
ACATGGTCCAGCCAAAAACATGGAGTTTGATGCTGTTGCAACAGCACCCT12.941176470588235No Hit
CTCTAGGGTCTCTCCTACTAAGTTCTATAGTAACAACTGATACAACATCA12.941176470588235No Hit
CCCAAGGCCCTGGAATTGCAAGCAGAGTATCAGTACCCAAGAATGCTAGG12.941176470588235No Hit
GTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCACAAAAAGAAA12.941176470588235No Hit
CAAAGAAAAAAACTAAAATAAAATTTAAATAAGAACATAAATATAAAAAA12.941176470588235No Hit
GACGAAGTAGAATAATCTTGCACATAGGGAATATAATCTAAATATGGATT12.941176470588235No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC12.941176470588235No Hit
TCTCTGCACACACACATCACTTTGCCGGTATCAGGGTAACAGGAACATTC12.941176470588235No Hit
ATTGAGGATGGTTACAGGACTAAGGAACACTCCGTCCATTCAATCCAGAG12.941176470588235No Hit
TGTGTGCTTTTTTGATCCGCTGCATAGCCTGATCCCTGTTCATTCTGATG12.941176470588235No Hit
GAAAAAAGGTCCTGCATTCCAAGTGAGAACATGAAATAAAGGGCTCTCTT12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)