FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154261
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT56620.49053030467112724No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA26180.2268117869355371No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT25180.21814823510453873No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC23690.20523954287635118No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG22760.19718243967352272No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21490.1861797288481548No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT21350.184966831591815No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20340.1762166442425067No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT19380.16789963448474826No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT19270.16694664378333843No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA19100.16547383997206871No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT17830.1544711291467008No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA16330.14147580140020324No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA16220.14052281069879344No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT16040.13896337136921372No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA15830.13714402548470406No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT15450.13385187578892468No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC15030.13021318401990536No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG14900.1290869222818756No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG14660.12700766984243597No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14350.12432196877482649No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13970.12102981907904711No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT13930.12068327700580717No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA13880.12025009941425725No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG13760.11921047319453745No Hit
GTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCTGTATCAGTCT13440.11643813660861797No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG13050.1130593513945286No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC12750.11046028584522911No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG12690.10994047273536921No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT12630.10942065962550931No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG12340.10690822959451979No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11900.10309626678888051No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA11830.10248981816071061No Hit
ATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACATAC11700.10136355642268083No Hit
GTATCAGTCTCCACGTTTAATTTGACAAAAGGTCTTTTTGCTGTGTAACT11600.10049720123958099No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGT100.007087451145.024291
GTCTTAC100.007088369145.018023
GGTACCG100.007091124144.99916145
GTAACAA14800.0124.441154
GAAACAC4750.0120.593934
AAACACG4800.0117.827145
CACGCAC1150.0113.4923553
GCGACGA203.8642992E-4108.763512
TAGAAAC5450.0106.435242
AGCACGC1300.0100.401441
GCACGCA1300.0100.3970952
CGCACTT1300.0100.3970955
CACACCG406.11828E-790.62448145
GGCGACG259.380868E-487.014581
TAGTAAC21400.086.4009252
AACACGT2100.082.867446
ACGCACT1700.081.039484
ACGTGCA3850.080.984092
CAGAGCG451.2320961E-680.565575
TAACAAG23050.080.5306245
Produced by FastQC (version 0.11.5)