FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039272
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT30720.29559152945523404No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26030.2504637861887937No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17680.17011908335835085No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17170.1652118021076292No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA16000.15395392159126775No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG13590.13076461215158303No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13590.13076461215158303No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC13310.12807041852373582No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG12960.12470267648892687No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA12900.1241253492829596No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA12890.12402912808196506No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA11680.11238636276162545No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG11590.11152037195267456No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT11290.10863373592283829No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA11220.10796018751587651No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT11130.10709419670692562No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT10940.10526599388802932No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT10890.1047848878830566No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC10830.10420756067708935No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG10750.103437791069133No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA10510.101128482245264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC100.0070860214145.03344
GAGGTTG100.0070860214145.03345
CTCGGGC100.0070860214145.03343
GAGGCTC100.00709112144.99852145
GTAACAA12450.0127.5594944
GAAACAC4650.0123.200424
CACGCAC1700.0115.1735843
TAGAAAC5100.0113.7516862
AAACACG5250.0110.501635
CATAAGG352.7572423E-7103.600271
AGCACGC1900.0103.055011
GCACGCA1900.0103.050052
CGCACTT1900.0103.050055
AACACGT1950.096.6889346
ACACGTG2000.094.249037
ACGCACT2200.088.9977654
TAACAAG18050.087.984255
TAGTAAC20650.076.555162
AACACGA5300.075.2531746
CCTTATG608.019197E-872.516694
Produced by FastQC (version 0.11.5)