FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172931
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG40410.3445215447455989No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC39700.33846833274932625No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA35400.30180803474373175No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG31570.2691547925666557No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT24680.21041305925071468No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA22080.18824636743337844No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA20930.17844186912955665No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA20830.1775893040596591No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT20190.1721328876123148No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT19840.16914890986767336No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC19470.16599441910905244No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC19310.1646303149972164No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT17190.14655593551538837No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT16770.1429751622218187No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT16760.1428899057148289No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC15500.1321475858341198No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA15290.130357199187335No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT15150.1291636080894784No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT15140.12907835158248865No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG14890.12694693890774478No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC14090.12012641834856441No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT13780.117483466631882No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA12890.10989563750979385No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA12810.1092135854538758No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT11840.10094370427586959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC14400.0120.344621
GCACGCA14800.0119.051752
CGCACTT15750.0115.0934755
ACGCACT15450.0114.512414
CACGCAC16150.0109.100073
GCACTTC10550.084.536436
GAAGCGG6750.075.1944051
GCGGAGT4050.073.404066
CACTTGA11000.073.158717
GCGGTGC8550.072.084861
CTTGATT11250.071.532959
AAACACG2900.070.008585
CCCCGTT5250.066.285369
CGCACGC350.003564925462.150481
CCCCCGT5500.061.954228
GCACTTG14850.058.5930376
CGGTGCG2850.058.5159452
CACTTCG2050.056.5850687
ACTTCGG1950.055.7689368
GCACCAC26800.054.652226
Produced by FastQC (version 0.11.5)