FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083140
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33230.306793212327123No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT25910.23921192089665233No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA21990.2030208467972746No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC18710.17273851948963198No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG17030.15722805916132726No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16840.1554738999575309No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT15800.14587218642096128No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15700.14494894473475267No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC15170.14005576379784698No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14770.13636279705301252No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT14710.13580885204128737No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT14180.1309156711043817No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA13550.1250992484812674No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12890.11900585335229057No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12870.11882120501504885No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG12500.11540521077607695No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT12260.11318943072917628No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT11980.11060435400779216No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT11730.10829624979227061No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG11350.10478793138467789No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC11020.10174123382018946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA12800.0126.325374
GCACGCA2000.0119.6408842
AAACACG6050.0117.4536065
CACGCAC2050.0116.722823
AGCACGC2100.0113.94371
GAAACAC6400.0111.030374
TAGAAAC7050.0100.7935262
AACACGT2050.095.500496
CGCACTT2650.093.031225
AACACGA5750.090.794666
TAGTAAC18300.089.1511842
GAAGCGG1750.087.011561
ACACGTG2250.086.9995047
TAACAAG19100.084.657835
ACACGAG6200.084.193067
ACAACAG2100.079.4153061
CATAAGG755.638867E-1177.3436051
ACGCACT3200.077.041484
ACGTGCA3350.075.7563252
CCTTATG502.3045523E-672.509634
Produced by FastQC (version 0.11.5)