FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080553
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT36750.34010363212170064No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29770.2755070783200824No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA19300.178612247617655No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC18790.17389244211066002No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17900.16565591877492358No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17130.15852993791142128No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA15700.1452959734506313No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG14890.13779981176305095No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA14820.13715199532091438No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT14800.13696690490887536No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT14660.13567127202460222No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14460.133820367904212No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG13410.12410312127216343No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA13120.12141931029759762No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12810.11855040891099279No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT12200.11290515134380268No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC12070.11170206366554902No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG11880.10994370475117833No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT11310.10466862800806624No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT11190.10355808553583212No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA11140.10309535950573456No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC11060.10235499785757847No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT11040.10216990744553946No Hit
GTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCTGTATCAGTCT11030.10207736223951994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGA100.00708624145.032171
GCAGCGC100.00708624145.032171
CATAGGT100.007091144144.99861145
GTACTCG100.007091144144.99861145
GTAACAA13050.0123.9163744
GAAACAC6150.0116.733214
CACGCAC1750.0116.0257343
AAACACG6450.0111.303765
TAGAAAC6750.0107.431242
AACACGT2200.0105.477946
AGCACGC1950.0104.1256561
GCACGCA2000.0101.5225142
ACGTGCA4200.088.0552442
TAGTAAC18450.087.648172
GGGGTAC259.378686E-487.01931
TAACAAG19000.085.874315
ACACGTG2750.084.362837
CGCACTT2600.083.67245
AACACGA6200.080.703386
ACACGAG6300.079.4047
Produced by FastQC (version 0.11.5)