FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045826
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT34820.33294257362123336No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC34780.33256010081983045No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25220.2411491012845349No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA24400.23330840885577522No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG20250.1936268557102233No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT19870.18999336409689566No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT19570.18712481808637382No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT19340.18492559947830708No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18110.1731645608351676No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA15940.15241541135905973No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT15800.15107675655414954No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA15570.1488775379460828No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT15110.14447910072994935No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG14450.13816829950680132No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA13820.1321443528847055No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC13330.12745906106751984No Hit
AGCACAGCTAATGATAACATATGATACACCAAAGATGTGGGAGATGGGGA12970.12401680585489364No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT12960.12392118765454292No Hit
GTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC12730.12172196904647618No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT11790.11273385821350779No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA11670.11158643980929907No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT11580.1107258760061425No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG11570.11063025780579179No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG11490.10986531220298597No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG11380.10881351199912795No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA11270.10776171179526996No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC11140.1065186751907105No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA10960.10479754758439741No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT10750.10278956537703213No Hit
GAATAGAGGGGACCCTTTCCTTACTTCGATGAAATTTTCTCCTCCTCCCT10620.10154652877247268No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC10600.10135529237177121No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC10540.10078158316966683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC5400.0123.535453
AGCACGC5550.0120.202411
GCACGCA5600.0119.123482
CGGTGCA256.4606047E-6116.0159152
GAAACAC3150.0115.0951464
CGCACTT6300.0110.491345
AAACACG3300.0109.863565
ACGCACT6300.0105.8875354
TAGTAAC18050.087.574342
GTAACAA18200.086.852584
TAGAAAC4250.085.3058242
GCGGTGC1200.084.5989761
AACACGA3300.083.496316
GTAGTAA19750.081.508751
AGTAACA19950.079.2339253
ACGTGCA1500.072.509942
GCCCGCA300.001934714472.509942
CCCGCAC300.001934714472.509943
ACACGAG3850.069.674897
CGGTGCG651.3967838E-766.932262
Produced by FastQC (version 0.11.5)