FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169386
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT46190.3949936120322973No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG35870.30674217067760345No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT28940.24748030162837592No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT27360.23396893754500225No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG24790.21199159216888178No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA24470.20925511336718586No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23860.20403870065145296No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT23300.1992498627484851No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA22790.1948885996582822No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT21840.1867646782157474No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG21740.18590952859021745No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA21140.18077863083703755No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG20590.17607530789662268No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG20450.1748780984208807No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA20270.17333882909492676No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC19200.16418872810175594No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG19180.16401769817664996No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19030.162734973738355No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC18410.15743304606006914No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA18130.15503862710858518No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC17590.1504208191307233No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA17290.14785537025413337No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA16690.1427244725009535No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16220.13870526926096258No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA15950.13639636527203167No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT15830.13537018572139567No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA15480.13237716203204075No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA15410.13177855729416976No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA15340.13117995255629877No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA15310.13092340766863977No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT15170.1297261981928978No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT15160.1296406832303448No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA14960.12793038397928486No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14800.12656214457843687No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14570.12459530043971793No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA14550.12442427051461194No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT13780.1178396183980311No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG13770.1177541034354781No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA13610.11638586403463014No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA13470.11518865455888817No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT13310.1138204151580402No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA13240.11322181042016921No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG13080.11185357101932125No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG13030.11142599620655627No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT12970.11091290643123829No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA12910.11039981665592027No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA12900.11031430169336728No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA12890.11022878673081428No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA12880.11014327176826129No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT12550.10732127800401237No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT12270.10492685905252842No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT12120.10364413461423345No Hit
GATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAATGACATCATTCT11880.1015917755129615No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT11780.10073662588743151No Hit
TGCTAATACTGCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTT11750.10048008099977253No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG100.0070866705145.029721
GTAACAA17900.0131.255954
CACGCAC2350.0129.601033
GCACGCA2450.0124.311192
AGCACGC2500.0121.8249741
CCTTATG301.1004886E-7120.858114
GAAACAC6450.0115.798934
AAACACG6800.0110.905085
GGCTGGC203.863071E-4108.772293
TAGTAAC21850.0106.532132
TAACAAG22500.0104.743695
TAGAAAC7900.099.134242
CGCACTT3100.098.245945
ACGCACT3200.095.175754
AGTAACA25550.092.523863
GAGAAGT259.379509E-487.017834
ACAAGAG27100.086.945737
CTGTCCG353.437671E-582.856415145
GAAGCGG2650.082.09231
GAAGCAC7750.074.854051
Produced by FastQC (version 0.11.5)