FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143342
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC43200.3778397015066358No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT36830.32212583811318046No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA35580.3111929763797709No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26910.2353626473968419No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT25630.22416739698183047No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21970.19215597782640714No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA21420.18734551866370694No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT21080.1843717802722195No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT19090.16696666439263141No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG18640.16303083416860398No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA18360.16058187314032024No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG17270.15104841770878705No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16990.1485994566805033No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC16490.14422631198713945No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT15930.13932838993057195No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC15690.13722928047775731No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG14520.12699612189528592No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT14350.12550925269954222No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG14080.12314775456512574No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT13990.12236058852032027No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC13950.12201073694485115No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG13840.1210486451123111No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA13760.12034894196137289No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT13570.11868714697789463No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC13360.11685042620668182No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT13030.11396415070906167No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC12990.11361429913359258No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT12390.10836652550155595No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG12170.10644234183647587No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG11900.1040808437020594No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG11650.10189427135537747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTG100.0070901043145.006093
GCACGCA7600.0123.064382
AGCACGC7550.0121.958751
CACGCAC7900.0118.391043
CGCACTT10100.096.915
ACGCACT10200.093.1166464
GAAACAC3800.089.674824
AAACACG4000.085.191075
GCGGTGC1750.082.860621
GTAACAA17400.075.836524
TAGTAAC18300.073.691612
TAACAAG18450.071.5206155
AACACGT1450.070.002936
ACAACGC2600.069.711418
GAAGCGG1700.068.238151
CAACGCA2700.067.129519
AGTAACA20300.066.788523
TAGAAAC6150.063.6612052
AACACGA3900.063.2077756
ACGTGCA2800.062.1454662
Produced by FastQC (version 0.11.5)