FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248857
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT36350.2910661508883723No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT29340.2349348244034345No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27030.216437910825659No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC20650.16535119713465993No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT19930.15958592537015848No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT19800.15854497352379016No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG19370.15510182510887954No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA19060.15261955532138588No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT18790.15045757840969784No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC18330.146774210337933No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC18230.1459734781484189No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC17750.14212996363875127No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC17230.13796615625327802No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT16420.13148022551821387No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC16320.13067949332869977No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT15810.12659575916217788No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG15720.1258751001916152No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA15300.12251202499565603No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA15210.12179136602509336No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC15130.12115078027348208No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT14390.1152253620710778No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC14280.11434455666261228No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG14150.11330360481624398No Hit
AACCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATG13730.10994052962028479No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA13490.10801877236545096No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT13380.10713796695698548No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT13350.10689774730013124No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA13290.1064173079864228No Hit
GTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC13260.10617708832956856No Hit
TCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAGATTTCTTTCT13140.10521620970215165No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC12720.10185313450619246No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG12550.1004918897840185No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG100.0070902593145.005552
GCACGCA4150.0127.535022
AGCACGC4100.0127.321961
CACGCAC4300.0123.086113
GCGTTGA203.865695E-4108.754174
CGCACTT5350.0104.34985
ACGCACT5350.0100.2842254
AAACACG3450.098.7719045
AACACGA2600.094.8113256
GAAACAC3700.092.098134
ATATAGG1800.080.558653
TAGAAAC4250.080.179552
ACACGAG3150.078.253847
GTAGTAA11600.073.752841
GGTTAGG300.001935560672.5027851
GTACACT406.6625704E-572.502784
TTTCCCG809.822543E-1172.49988145
CGGTGCC554.0612704E-665.911622
TAGTAAC13050.065.0024952
CAACGCA1450.064.99999
Produced by FastQC (version 0.11.5)