FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085462
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT37220.3428954675520654No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG25170.23188282961540801No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT22360.20599523520860244No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA18690.1721847471399275No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG18600.17135560710554584No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG18350.16905244034337452No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17050.15707597318008368No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA16840.1551413130998598No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT16260.14979796621162234No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA15790.14546801269874027No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC15720.1448231260053323No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15480.14261208591364782No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA14690.13533407894518648No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT14660.1350576989337259No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA14630.13478131892226536No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC14510.13367579887642314No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT14480.13339941886496257No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT14180.130635618750357No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA14100.12989860538646217No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG13460.1240024984753036No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13190.12151507837215858No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13030.12004105164436893No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT12850.11838277157560559No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG12490.1150662114380789No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA12340.11368431138077612No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA12190.11230241132347332No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA12100.11147327128909164No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA12100.11147327128909164No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT11930.10990711789081516No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT11910.10972286454984145No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT11870.10935435786789405No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG11830.10898585118594663No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA11800.10870947117448608No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11780.10852521783351236No Hit
TTTGAGAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC11770.1084330911630255No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT11370.1047480243435514No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC11170.10290549093381436No Hit
TAGTTAGGACTTCTGTTTGTGGATTATAGGAAAATAGAGGGGACCCTTTC11100.1022606042404064No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG11030.10161571754699841No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT10960.10097083085359045No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT10960.10097083085359045No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAT100.0070910254144.99945145
CACGTGA100.0070910254144.999458
GTAACAA13150.0127.9200444
CACGCAC1300.0122.703143
GCACGCA1350.0118.158582
GAAACAC5550.0117.577954
AAACACG5650.0115.4969255
AGCACGC1450.0110.009721
GAGCAGA700.0103.580581
TAGAAAC6450.0103.419992
TAGTAAC16600.0101.334242
CATAAGG950.099.21931
AACACGT2300.094.573576
TAACAAG18650.090.195635
AGTAACA20000.085.195023
GAAGCGG1450.085.007511
CGCACTT1900.083.954795
AACACGA5250.082.864466
ACAAGAG22000.076.4542547
ACACGTG2850.076.31557
Produced by FastQC (version 0.11.5)