FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931104
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT24170.25958432140770527No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17930.19256710313778053No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT15530.16679124995704026No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT14160.15207753376636765No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC13650.14660016496546036No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA13590.14595576863594184No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT13100.14069319861154073No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13070.14037100044678147No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC12850.13800821390521362No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA12520.1344640340928618No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC12110.13006065917448534No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA11740.12608688180912123No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG11500.1235092964910472No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG11000.11813932707839296No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10980.1179245283018868No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10970.1178171289136337No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT10870.11674313503110287No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG10850.1165283362545967No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA10530.11309155583049799No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC10530.11309155583049799No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG10520.1129841564422449No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT10470.11244715950097948No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC10400.1116953637832079No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC10390.11158796439495482No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT10370.11137316561844862No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG10090.10836598274736227No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT9980.10718458947657834No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA9940.10675499192356601No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC9880.1061105955940475No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT9860.10589579681754133No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT9830.10557359865278207No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA9390.10084802556964635No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT9380.10074062618139328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGA100.007088602145.014941
GATCGTG100.0070908787144.999367
GTAACAA9550.0128.311654
CACGCAC850.0119.4240653
AAACACG3400.0119.4240655
AACACGT1550.0116.9475256
GAAACAC3650.0111.244334
GCACGCA1050.096.676622
ACACGTG1950.092.948317
TAGAAAC4700.092.562712
AGCACGC1150.088.269951
TAACAAG14350.085.392065
ACGTGCA2000.083.383582
CAACGCA755.638867E-1177.3329859
TAGTAAC16300.076.066112
CATAAGG300.001934909572.507471
AACACGA3200.072.507476
AACGTAT702.8103386E-972.507466
CGGTGCG502.304705E-672.507462
GAGCGTT406.6630935E-572.499687
Produced by FastQC (version 0.11.5)