FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054219
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT91570.8686051000788262No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA35540.33712160376544154No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG26640.2526989173976185No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG22300.2115310006744329No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20500.19445674949891814No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20430.19379275084209258No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT19360.1836430570877588No Hit
AGCACGCACTTTCTGTCTTCTAAAGGGCCGCCACATGCCAGGAGCTCAGG17540.16637909201029388No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA15580.1477871296191778No Hit
ATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCT15270.14484656413895025No Hit
CATGTTAACTTTTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG15220.14437227938407485No Hit
CAAAAGGATTGGCCGATGATTACTTTTGGAAAAAGAAAGAAAAGCTGGGA14920.14152657085482237No Hit
CATTTCAACTTCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG14310.13574029684534236No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA14000.13279973136511486No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA13910.1319460188063391No Hit
CAACCAAGACTACTCGTTAAGTAATGAATCCTCATTGGATGAGGAAGGGA13700.12995402283586238No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12950.12283975151273123No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT12200.11572548018960006No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA12150.11525119543472466No Hit
GTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTGATTGA11890.11278491470937252No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC11730.11126720349377121No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA11610.11012892008207024No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC11550.10955977837621975No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC10990.10424778912161514No Hit
GGGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACC10920.10358379046478958No Hit
TACAAGTACACAGATTTTTCCCTCCCACTCACAAATAGGGAGCCAATCCT10750.10197122229821318No Hit
GTGCAAGACAGGCTGTGTGGTGTACCCACGCCTGTACAGCTTTACACATA10720.10168665144528793No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC10560.10016894022968663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC21300.0123.5563351
GCACGCA21450.0123.03032
CACGCAC22450.0118.5189363
CGCACTT25000.0108.755
ACGCACT25750.0104.456314
GCACTTT25000.096.866
GTAACAA7500.088.9333344
GCACTTC7650.088.137256
TTCGCGG259.386891E-487.02
AAACACG2100.082.857145
CAACGCA4050.076.975319
GAAACAC2300.075.6521764
AACACGT1450.075.06
TAGTAAC9050.072.900552
AACAACG4550.070.109897
ACAACGC4600.067.771748
GAAGCGG5000.066.71
ACACGTG1650.065.909097
TGCGCTC554.061758E-665.909095
TAACAAG10750.062.720935
Produced by FastQC (version 0.11.5)