FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137385
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT29870.2626199571824844No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT27570.24239813255845644No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC27330.24028802911942745No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT25250.2220004659811761No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT24790.21795610105637053No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA21180.1862166284943093No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA16710.14691595194239418No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA15850.13935474795254027No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA15350.13495869912122982No Hit
AGCACAGCTAATGATAACATATGATACACCAAAGATGTGGGAGATGGGGA14910.13109017614967666No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT14690.12915591466390008No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC14270.12546323364559933No Hit
GTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC13730.12071550090778409No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT13720.12062757993115787No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG13480.11851747649212889No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC13460.11834163453887646No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT13280.1167590569596047No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC13280.1167590569596047No Hit
GAATAGAGGGGACCCTTTCCTTACTTCGATGAAATTTTCTCCTCCTCCCT13100.11517647938033294No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC13080.11500063742708054No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG12970.11403350668419225No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC12910.113505980824435No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC12700.11165964031528462No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT12280.10796695929698387No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG12180.10708774953072178No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC12040.10585685585795487No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA11940.10497764609169277No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11750.10330714753579483No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC11630.10225209581628034No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG11480.1009332811668872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC6100.0127.193583
GCACGCA6150.0126.159482
AGCACGC6100.0126.004851
CGCACTT6750.0116.0195545
ACGCACT6850.0114.3258364
GAAACAC4050.0109.215944
CGGTGCC750.0106.351272
AAACACG4300.0102.866185
TAGAAAC5250.085.633482
GCGGTGC1300.083.6679461
CGGAGCG353.4346063E-582.871123
ACAACGC703.092282E-1182.8565448
TAGTAAC17950.082.005472
GTAGTAA18100.081.726481
AACACGA3650.075.492186
GTAACAA19850.074.886684
GCACTTT9550.074.4104466
ACGTGCA2050.074.2808152
AACACGT2350.074.055046
AGTAACA20500.073.219663
Produced by FastQC (version 0.11.5)