FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237067
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT38410.31049247938874774No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT24650.1992616406387043No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT22250.1798609129497432No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT20000.16167273074134222No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG19700.15924763978022208No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA18820.15213403962760302No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG18230.14736469407073344No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT18190.14704134860925075No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18000.145505457667208No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17690.1429995303407172No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA17490.14138280303330378No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17370.14041276664885574No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC17190.13895771207218363No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC17090.13814934841847692No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17000.1374218211301409No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA16450.13297582103475397No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA15760.12739811182417768No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT15610.1261855663436176No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT15170.12262876626730809No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT14560.11769774797969713No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT14450.11680854796061975No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA14310.11567683884543035No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT14240.11511098428783567No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA14090.1138984388072756No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA14040.11349425698042223No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA13910.11244338423060353No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG13910.11244338423060353No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC13870.11212003876912083No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG13630.11017996600022471No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG13620.11009912963485405No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA13310.10759320230836326No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT13260.1071890204815099No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG12880.10411723859742439No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG12810.1035513840398297No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT12630.10209632946315762No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC12580.10169214763630426No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT12470.10080294761722688No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA12460.10072211125185619No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA12420.10039876579037352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCACG100.0070911357144.99951145
GTAACAA14150.0131.176244
AGCACGC1500.0130.510121
CACGCAC1550.0126.30013
GAAACAC5900.0120.433074
GCACGCA1650.0118.645562
AAACACG6350.0111.898445
TAGAAAC6600.0108.758432
CGCACTT2100.093.2215045
TAACAAG20850.089.371445
TAGTAAC21500.086.332272
ACGCACT2300.085.1152954
AACACGT3350.082.245186
CGGTGCG451.2324927E-680.56182
AACACGA5800.076.255916
AGTAACA25400.074.218353
CATAAGG502.3055018E-672.5056151
ACACGAG6200.071.330417
CGGAGCA1350.069.820232
ACAAGAG27800.067.805537
Produced by FastQC (version 0.11.5)