FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n01_DARPA_FLUB20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n01_DARPA_FLUB20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143990
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT31680.2769254976005035No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT29660.2592680005944108No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20490.1791099572548711No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC19710.1722917158366769No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT18000.1573440327275588No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA17260.15087544471542583No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC16980.14842787087299714No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT16790.14676701719420626No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT16540.1445816834063235No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC16050.14029842918207328No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA14390.12578781283053173No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC14200.12412695915174084No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG14040.12272834552749586No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC13780.1204555983880978No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT13630.11914439811536814No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG13490.1179206111941538No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG13420.11730871773354662No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC13080.11433666378202606No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT12950.11320029021232704No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT12910.11285063680626578No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA12870.11250098340020455No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC12860.11241357004868922No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC12730.1112771964789902No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT12630.11040306296383709No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA12580.10996599620626053No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA12440.10874220928504619No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC12220.10681911555170936No Hit
GTATTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC11590.10131207440624482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA100.007084747145.042761
CTCAACG100.007085673145.03645
TCCTAGG100.007085673145.03643
CGAGCGG100.0070912316144.99838145
CACGCAC3050.0126.015253
AGCACGC3250.0118.265631
AGCCCGC256.4558026E-6116.034211
GCACGCA3400.0115.175972
GCTACGA203.8616688E-4108.782071
CGCACTT4300.099.501735
GAAACAC3450.096.690954
AAACACG3450.096.690955
GCCCGCA301.5975646E-596.690952
ACGCACT4250.092.1407854
TAGAAAC4300.082.637032
AACACGA2650.076.623016
TTTCCCG1150.075.65133145
AACACGT1750.074.590166
GTAGTAA11550.074.091111
GGAGCGT502.3030134E-672.518223
Produced by FastQC (version 0.11.5)